![]() ![]() Layne adheres to the “plateau” theory of HPLC advancement. So many choices exist and before they buy anything they should see what works best for their applications.” Rather than telling customers ‘buy this!’ we present them with alternatives. “All these technologies have a role to play. “Every major HPLC system manufacturer offers both HPLC, UHPLC, and mid-pressure systems, both in fully porous and core-shell platforms,” says Jeff Layne, senior technical marketing manager at Phenomenex, a provider of HPLC columns. ![]() ![]() Unlike product sectors where companies grow but only at the expense of the overall market, HPLC is and remains a growth industry where rising tides raise all ships: A recent research study estimated. To reduce peak dispersion on older instrument platforms manufacturers have eliminated unnecessary volumes through greater precision of fittings and connectors, and by reducing column diameters. HPLC reliability and robustness have improved across the board, at every level, while performance has held steady or improved. In 2015, Waters introduced the ACQUITY Arc UHPLC system, which employs a selectable dwell feature to enable users to easily replicate established HPLC methods, independent of the system of origin, while preserving the profile of their method.Įxisting on a technologic plateau is not the same as standing still. Nevertheless, since the advent of U(H)PLC, customers have been concerned with the analytical transfer of methods from older platforms to newer ones. They usually adopt more modern technologies for new products.” “Organizations are unlikely to invest in altering methods that they have been running for 15 years or more. Methods developed for a particular analyte on a specific instrument type tend to stay put, although analysis sometimes develop methods on higher-throughput UPLC and transfer them to HPLC for routine use. Very little crosstalk occurs among HPLC platforms. Yet traditional HPLC Systems still remain a very large part of the analytical laboratory, particularly in routine analysis and QA/QC,” Grumbach tells Biocompare. “When we launched UPLC in 2004, we aspired to replace HPLC technology over time. The best technology depends on the needs and workflows of a particular organization.”Īs high-pressure systems were gaining followers, Waters and its UHPLC competitors underestimated the pull of routine analytical methods for common products, particularly among highly regulated industries. And even with these higher-performing options many organizations continue to run legacy methods that utilize traditional HPLC systems with 5 micron particles. Other customers are quite happy with mid-tier instruments in the 9,000 to 10,000 psi range and superficially porous particles. “Some customers require the highest resolution that is only achieved with higher-end UPLC systems and sub 2 µm particles. “We’ve recognized, over the last several years, that needs exist for all levels of chromatographic performance in the pharmaceutical, biopharmaceutical, food, and materials industries,” notes Eric Grumbach, director, product marketing, chromatography systems at Waters. The best technology depends on the needs and workflows of a particular organization. Fused-Core, and its follow-on products (known as core-shell, superficially porous, porous-shell, etc.), promised performance approaching that of UHPLC but with much lower backpressures.Īdoption of mass spectrometers (MSs) as detectors expanded the capabilities of HPLC even further by enabling the discrimination of small mass variants, particularly in biomolecules. In 2006 Advanced Materials Technology (AMT) introduced Fused-Core ® technology, with particle sizes of between 2 and 3 microns. Eventually other major vendors developed similar products, generically known as UHPLC (ultra high-performance liquid chromatography). Waters’ 2004 debut of UPLC ® leveraged sub-two-micron (s2m) stationary-phase particles, low instrument dispersion, and pressures up to 15,000 psi. High-performance liquid chromatography (HPLC) has undergone a pattern of breakthrough technologic advances followed by periods of slower, incremental improvements. ![]()
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